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Supertechs/Rabbit anti-Calf TdT Immunoaffinity Purified/004

  • 产品编号: 004
  • 美  元  价: 待定
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司: Supertechs,inc
  • 产品分类:
  • 公司分类: TdT Detection
Supertechs/Rabbit anti-Calf TdT Immunoaffinity Purified/004
  • Supertechs/Rabbit anti-Calf TdT Immunoaffinity Purified/004
商品介绍

AnimmunoaffinitypurifiedreagentknowntoreactwithTdTfromcows,humans,rats,mice,guineapigs,cats,andchickens.ThisproductisobtainedfromrabbitserumafterimmunizationwithpureTdTantigenandsubsequentchromatographyonTdTcolumns.

Therabbitanti-calfTdTissuppliedat100-200 µgIgGpermLinPBSwith1%BSAand0.1%sodiumazide.Appropriatedilutionsforspecificapplicationsmustbedeterminedbytheindividualinvestigator.Forindirectimmunofluorescencetestsonslides,wesuggestusingthisreagentat10folddilutioninPBScontaining1%BSA.Aone(1)mLvialwillprovideenoughreagentfor500slidetests.Activityoftheproductismaintainedforatleastoneyearwhenstoredat20°C.

品牌介绍

Supertechs was established in 1982 in Rockville, Maryland for the purpose of producing monospecific antibodies to Terminal deoxynucleotidyl Transferase (TdT) and to provide these antibodies commercially. Human TdT is a naturally occurring nuclear protein found at a detectable concentration in prelymphocytes during early biological development. Abnormal or cancerous human hematopoietic cells may express an abnormal concentration of TdT independent of the developmental stage. Overpopulation of TdT positive cells in blood and bone marrow is symptomatic of a disease.
The availability of our immunological reagents specific for TdT determination in blood smear, bone marrow, and cell suspension enables researchers and clinicians to accurately and reliably analyze tissue.
Our website is exclusive to immunological reagents for TdT determination. If you have further questions regarding the availability of TdT enzyme or other related products, then please contact our company by phone or email.
General Info & Policies
Analytical and performance characteristics are not established for FDA Class I Analyte Specific Reagents. We are not liable for any damages caused by improper use of our products. All matters related to specific practical applications are the users’ responsibility.
Terms of payment are net 30 days.
Anti-TdT Reagents and Related Products
Supertechs produces immunoaffinity-purified monoclonal anti-TdT antibodies conjugated with fluorescein isothiocyanate (FITC) and their complementary negative control reagents for flow cytometric analysis.
As control cells for flow cytometry studies - Supertechs manufactures lymphoblastoid REH (TdT⁺) and RA-1 (TdT⁻) cells prepared as a fixed cell suspension with about 50% TdT⁺ cells and 50% TdT⁻.
For slide staining, immunoaffinity-purified polyclonal rabbit antibodies specific to human and bovine TdT as well as FITC labeled goat:anti-rabbit reagents are available. These conjugates are usually used for staining of TdT positive cells in blood or bone marrow smears and in paraffin tissue sections


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Supertechs的TdT控件

淋巴母细胞对照细胞和阴性对照试剂用于质量控制测试

人淋巴母细胞悬浮

使用Supertechs Cat#6600对Supertechs Cat#9500控制细胞悬浮液的染色。

9500 TdT控制单元

Supertechs Cat#9500是一种固定的(多聚甲醛)细胞悬浮液,其中包含具有稳定TdT抗原的人淋巴母细胞REH(TdT⁺)和RA-1(TdT⁻)细胞。这种细胞悬液可以用作TdT流式细胞仪测定的对照。对照细胞悬浮液在一个方便的滴瓶中含有约50%TdT 6和50%TdT 6细胞的混合物,浓度为2 x 10 6细胞/mL(1.0 mL,〜20次测试)。我们的稳定化细胞悬浮液在4°C下储存后,在制备后的保质期约为3个月。

对照细胞可用于在流式细胞术研究中设置阈值和门控,并可用于培训技术人员进行和评估TdT流式细胞术实验。 

对照抗体

003负控制

兔多克隆IgG

一种免疫亲和纯化的兔抗β-半乳糖苷酶(大肠杆菌)IgG,对细菌蛋白具有特异性,可用作与兔抗TdT试剂(例如我们的Cat#004)无关的抗体对照。该产品包含约110 µg免疫亲和力每毫升含1%BSA和0.1%叠氮化钠的PBS中纯化的兔多克隆IgG。可以以1:10的比例在测试载玻片上使用。

该试剂专门用作TdT玻片测试的阴性对照。

流式细胞仪结果显示TdT⁺T-ALL组织的染色极少。

由Elisabeth Paietta博士提供

8100 FITC阴性对照IgG₁

具有与细菌或酵母蛋白无关的特异性的小鼠单克隆同种型对照,可用作小鼠单克隆人类抗TdT试剂(如Cat#6100和Cat#6600)的阴性对照。该产品直接与异硫氰酸荧光素(FITC)偶联。

产物经过蛋白A纯化,在含1%BSA和0.1%叠氮化钠的PBS中以50μg/ mL的浓度制备。它可以与Supertechs Cat#9500对照细胞悬液按1:10稀释比例使用。

该试剂不适用于TdT测试载玻片。 

8200 FITC阴性对照IgG 2

具有与细菌或酵母蛋白无关的特异性的小鼠单克隆同种型对照,可以与Cat#8100一起用作小鼠单克隆人抗TdT试剂(如Cat#6100)的阴性对照。该产品直接与异硫氰酸荧光素(FITC)偶联。

产物经过蛋白A纯化,在含1%BSA和0.1%叠氮化钠的PBS中以50μg/ mL的浓度制备。它可以与Supertechs Cat#9500对照细胞悬液按1:10稀释比例使用。

该试剂不适用于TdT测试载玻片。


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