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Supertechs/FITC:Monoclonal anti-Human TdT Reagents/5500

  • 产品编号: 5500
  • 美  元  价: 待定
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司: Supertechs,inc
  • 产品分类:
  • 公司分类: TdT Detection
Supertechs/FITC:Monoclonal anti-Human TdT Reagents/5500
  • Supertechs/FITC:Monoclonal anti-Human TdT Reagents/5500
商品介绍

AsetofsolutionsusedforindirectimmunofluorescenceprocedurethatallowsforIlluminationofTdT⁺cellsbycombinedfluorescenceandphase-contrastmicroscopy.Allimmunologicalreagentsinthesetareprovidedinready-to-useform(includinganegativecontrol).Thereagentsareshippedatambienttemperaturebutcanbestoredintherefrigeratororfreezer.Thereagentsarestableforatleast6months.

 

ReagentsProvided:

Normalgoatserum........................................................(1.00mL)

Negativemonoclonalcontrol........................................(0.60mL)

Mouseanti-humanTdTmixture...................................(1.00mL)

FITC:Goatanti-MouseIgG.............................................(1.00mL)

Glycerol:PBSmountingmedium.................................(1.00mL)

品牌介绍

Supertechs was established in 1982 in Rockville, Maryland for the purpose of producing monospecific antibodies to Terminal deoxynucleotidyl Transferase (TdT) and to provide these antibodies commercially. Human TdT is a naturally occurring nuclear protein found at a detectable concentration in prelymphocytes during early biological development. Abnormal or cancerous human hematopoietic cells may express an abnormal concentration of TdT independent of the developmental stage. Overpopulation of TdT positive cells in blood and bone marrow is symptomatic of a disease.
The availability of our immunological reagents specific for TdT determination in blood smear, bone marrow, and cell suspension enables researchers and clinicians to accurately and reliably analyze tissue.
Our website is exclusive to immunological reagents for TdT determination. If you have further questions regarding the availability of TdT enzyme or other related products, then please contact our company by phone or email.
General Info & Policies
Analytical and performance characteristics are not established for FDA Class I Analyte Specific Reagents. We are not liable for any damages caused by improper use of our products. All matters related to specific practical applications are the users’ responsibility.
Terms of payment are net 30 days.
Anti-TdT Reagents and Related Products
Supertechs produces immunoaffinity-purified monoclonal anti-TdT antibodies conjugated with fluorescein isothiocyanate (FITC) and their complementary negative control reagents for flow cytometric analysis.
As control cells for flow cytometry studies - Supertechs manufactures lymphoblastoid REH (TdT⁺) and RA-1 (TdT⁻) cells prepared as a fixed cell suspension with about 50% TdT⁺ cells and 50% TdT⁻.
For slide staining, immunoaffinity-purified polyclonal rabbit antibodies specific to human and bovine TdT as well as FITC labeled goat:anti-rabbit reagents are available. These conjugates are usually used for staining of TdT positive cells in blood or bone marrow smears and in paraffin tissue sections


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Supertechs其他产品

二级抗体

用Cat#008,FITC:IgG处理的淋巴母细胞REH细胞(TdT⁺)和RA-1细胞(TdT⁻)的40倍成像。

008 FITC:山羊抗兔IgG

F(ab')制备

该试剂经过免疫亲和纯化(吸收了人类血清),具有高滴度,可用于需要低背景检测兔IgG主试剂的任何细胞化学程序。

该产品以浓度为100 – 500 µg / mL的浓度在含1%BSA和0.1%叠氮化钠作为防腐剂的PBS中提供。在-20°C下储存时,产品的活性至少保持1年。用于特定应用的适当稀释度必须由个别研究者确定。对于载玻片上的间接免疫荧光测试,我们建议以10倍稀释度在含1%BSA的PBS中使用该试剂。一(1)ml小瓶将为500张测试载玻片提供足够的试剂。

典型用途:

与Cat组合使用时的中等密度脂蛋白(idI)免疫荧光。#004或其他主要兔IgG试剂。

用Cat#5200处理的淋巴母细胞REH细胞(TdT⁺)和RA-1细胞(TdT⁻)的40倍成像。

5200 FITC:山羊抗小鼠IgG

该试剂经过免疫亲和纯化(吸收了人类血清),具有高滴度,可用于需要低背景检测兔IgG主试剂的任何细胞化学程序。

该产品以浓度为200-500 µg / mL的浓度在含1%BSA和痕量叠氮化钠的PBS中提供。用于特定应用的适当稀释度必须由个别研究者确定。对于载玻片上的间接免疫荧光测试,我们建议以10倍稀释度在含1%BSA的PBS中使用该试剂。一(1)ml的小瓶应为500张测试载玻片提供足够的试剂。建议的存储温度为-20°C。

典型用途:

与Cat组合使用时的中等密度脂蛋白(idI)免疫荧光。#004或其他主要兔IgG试剂。

Supertechs固定和通透性

Supertechs的Fix和Perm Set包含两种用于流式细胞仪分析之前的血细胞制备的溶液-溶液A包含软固定剂,而溶液B则使细胞渗透以进行试剂染色。该设备有助于终端脱氧核苷酸转移酶(TdT)的流式细胞术检测。TdT是一种不稳定的核抗原。当固定技术太强时,流式细胞仪中TdT的染色会变弱。Supertechs的F和P解决方案允许进行温和的固定和通透性程序,从而可以通过流式细胞术更好地检测TdT⁺细胞。 

1001 Fix and Perm Solutions – I(50个测试)

包括固定溶液和带有通透介质的封闭缓冲液,可进行50次测试。1 x10⁶电池/测试

使用Supertechs Fix和Perm Solutions和Supertechs Cat#6600在20倍放大下对Supertechs Cat#9500控制细胞悬浮液进行染色。

1002 Fix and Perm Solutions – II(200个测试)

包括固定溶液和带有通透介质的封闭缓冲液,可进行200次测试。1 x10⁶电池/测试


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